G8795
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 | Immunoblotting Total cell extract of A431 cells was separated on SDS-PAGE and blotted with Monoclonal Anti-GAPDH (Cat. No. G8795). The antibody was developed with Goat Anti-Mouse IgG, Peroxidase conjugate (Cat. No. A2304), and a chemiluminescent substrate. Lanes: 1. 0.2 μg/mL antibody 2. 0.05 μg/mL antibody 3. 0.025 μg/mL antibody 4. 0.0125 μg/mL antibody 5. 0.01 μg/mL antibody |
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G8795
Sigma
Monoclonal Anti-GAPDH antibody produced in mouse
~1 mg/mL, clone GAPDH-71.1, purified immunoglobulin, buffered aqueous solution
| Synonym: | Anti-G3PD, Anti-G3PDH, Anti-Glyceraldehyde-3-phosphate dehydrogenase |
Description
| Immunogen | rabbit GAPDH. |
| Physical form | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
Properties
| antibody form | purified immunoglobulin |
| clone | GAPDH-71.1 (monoclonal) |
| form | buffered aqueous solution |
| mol wt | antigen mol wt ~37 kDa |
| species reactivity | rat, chicken, human, turkey, mouse, bovine, canine, mink, monkey, hamster, rabbit |
| does not react with | prokaryotes |
| concentration | ~1 mg/mL |
| application(s) | immunoblotting: 0.025-0.05 μg/mL using A431 total cell extract |
| immunocytochemistry: suitable | |
| indirect ELISA: suitable | |
| microarray: suitable | |
| isotype | IgM |
| Gene Information | human ... GAPDH(2597) |
| rat ... Gapdh(24383) | |
| mouse ... Gapdh(14433) | |
| shipped in | dry ice |
| storage temp. | −20°C |
Safety
| WGK Germany | 3 |
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References
| reference | Abel, E., et al., Mitochondrial fusion and function in Charcot-Marie-Tooth type 2A patient fibroblasts with mitofusin 2 mutations. Exp. Neurol. 211, 115-127, (2008) Abstract |
